Recombinant Protein Human Granulysin, GNLY
Discover a Recombinant Protein Human Granulysin
GNLY is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells (CD8), which are released upon antigen stimulation.
GNLY is an antimicrobial protein that kills intracellular pathogens, active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. It creates holes in the target cell membrane and destroys it. It is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. Kills Mycobacterium tuberculosis.
Characteristics
NCBI GENE ID: 10578 UniProtKB/Swiss-Prot: 22749 Location: Chr 2, 85.91 – 85.93 Mb PDB ID: 1L9L
Presentation: Stored in PBS at -80ºC
Source: E. coli
Molecular weight: 11 KDa, plus His-Tag
Buffer: PBS.
Lot Specifications
1. Concentration: lot specific
2. Total quantity per aliquot: see references
3. Storage: should be kept frozen at -20 or -80 degrees.
4. Applications:
a. Apoptosis research.
b. Antimicrobial peptides.
c. As calibrator or controls of detection kits.
5. Observations: Avoid freeze thaw cycles.
References
“Phenotypic and functional evaluation of CD3+CD4−CD8− T cells in human CD8 immunodeficiency” Iván Bernardo, Esther Mancebo, Ignacio Aguiló, Alberto Anel, Luis M. Allende, Juan M. Guerra-Vales, Jesús Ruiz–Contreras, Antonio Serrano, Paloma Talayero, Oscar de la Calle, Cecilia Gonzalez-Santesteban, Estela Paz-Artal, Haematologica. 2011 August; 96(8): 1195–1203
“Granulysin cristal structure and a structure-derived lytic mechanism” Anderson DH, Sawaya MR, Cascio D, Ernst W, Modlin R, Krensky A, Eisenberg D, J.Mol.Biol. (2002) 325 p.355
